Goodjob-Capture Software-operation progress
捕捉软件的操作流程
1:CEL image capture 化学发光影像的捕捉
The system will automatic active the image capture program when booting.
系统启动时，系统自动运行影像捕捉程序。
2:Quick user guide 快速使用指南
(1) choose Loop to start realtime image to show sample area and load chemi-membrance.
选择“Loop”键，实时影像会显示样品面积和负载化学膜。
(2) Choose bin2 to speed up realtime image running. 选择bin2，加快实时影像运行
PS: For chemi-sample pre-set parameter 备注：化学发光样品预先参数设置
1) auto-stretch atuo-stretch（自动延伸）
2) negative negative(否定、负的)
(3) turn off the reflectant white light when start to capture image 当开始影像捕捉时，关闭反射白光
(4) change exp.time 改变曝光时间
(5) press Loop again to stop realtime image .press Time-lapse,set desire capture number
再次按“Loop”键停止实时取相。按“Time-lapse”(延时)，设置想要的捕捉图片数量。
(6) choose directory to save capture images save as tif.must be key in this eaction is only first time use time-lapse. Routine use just press Next dir will auto save to new diretory.
选择目录，以tif.的后缀名保存照片。这一步在**次使用“Time lapse”时是非常关键的。常规应用就可以直接选择“Next dir”(下级目录)，系统会自动保存到新的地址。
PS:Must select “Autosave images”before capture. Otherwise the present directory will have no image.
备注：捕捉前必须选择“Autosave images”，负责目前的目录里不会存有图像。
(7) start to capture image Time-lapse fuction. 开始用“Time-lapse”捕捉图像。
(8) Under auto-stretch control in the beginning the display window will be high sensitive on weak bands and its image will be a little rough that depend on samples concentration.
在开始“auto-stretch”功能的作用下，窗口会出现高敏感度的弱信号条带并且图像有点粗糙，这都取决于样品的浓度。
This is helpful for user to know whether weaks bands is there. It will show nice picture in the final images after more fluorescent signal collected.
这有助于使用者发现是否存有弱信号条带。并且在收集更多荧光信号后，*后的成片效果会非常好。
Final images will display good signal intensity and have white and smooth background. This is because system has capture enough signal after Time-lapse choose final images as your best results.
*终成像会有好的信号强度和白色稳定的背景。这是因为“Time-lapse”，系统捕捉了足够多的信号。选择*后的图像作为你*好的结果。
(9) Press reset to next time capture 选择“Reset”（重设）开始下次捕捉
De-select Auto-save images when fininsh time-lapse
当 “time-lapse” 结束时，取消“Auto-save images”(自动保存图像)
De-select Auto-stretch 取消“Atuo-stretch”
Set desire 设定期望值
Black no. 黑度
White no. 白度
Will show image intensity increase gradually. 图像强度逐渐增加
(10) stop Loop. Start “Time lapse” 停止“Loop”。开始“Time-lapse”
(11) increase exp.start 增加曝光时间
During Time-lapse capture user can add any no. of imges or expand exp. Time before the last image reach. If you feel sample is too weak.You can stop any time.If you feel sample signal is too strong then just few images is enough.
在“Time-lapse”时，捕捉*后图像前使用者都可以增加图像数量或者改变曝光时间。如果你感觉样品信号太弱，任何时间都可以停止。如果你感觉太强，少量几张图像就够了。
Under non-auto-stretch control user can see sample signal increase one by one.this will look like X-ray film,but for quantitation user must take care which bands will easy to over expose and saturation.
没有选择“auto-stretch”功能的用户可以观察到样品信号一张张增强。这看起来像X光片。但是对于定量用户必须关心条带是否易于曝光和饱和。
User can choose any one image to check results after Time-lapse finished .Just click time-lapse no.
“Time-lapse”后，用户可以选择任何一张图像来核对结果。只需要点击对应的图像编码。
(12) Separate a group of Stack to images into individual image. 从一堆图像中分出一张独立的照片。
(13) Choose “Windows” “Titles” to spread each images on deskTop.
选择“window”-“Titles”，把每张照片放在桌面上。
(14) Collect all image into a stack file PS:“Stacks”- “imges to stack”-“name”
把所有的图像放在一个图像文件里。
(15) “Make montage”. PS:“Stacks”-“Make montage”
(16) Set scale factor 0.5* or 1* 设置“scale factor”（比例因子）0.5或0.1
(17) Quantitation: open one images 定量：打开一张图像
Familiar with the data of chemi-image:Row data should be black background.white bands and intensity will be positive.Most of lab whould like to see the image like X-ray film.so the data will be negative.it need to be inverted when chemi-image what to quantitation.It will not need to inverted.If original image capture with setting at Non-Negative.
熟悉化学发光图像的数据：并行数据应该是黑色背景。白色条带和亮度较大。大多数的实验室想观察X光片，所以数据应该是Negative.当化学发光图像要定量时，图像需要反过来。如果原始图像设置为Non-Negative，那么图像不需要翻转。
The follow image will see data is low when mouse on dark bands and data is high at white background.
当鼠标移动到黑色条带时，图像数据较低。白色条带数据较高。
Background correct
Input optimum Rolling ball radius 设置*佳“Rolling ball radius”值
(18) Choose quantitative tool draw around the target bands. 用定量分析工具（Image J）圈定分析条带
(19) Press“Analyze”“Measure”repeat for each bands. 选择“Analyze”-“Measure”对每条条带重复操作